Inhibition by bestatin of a mouse ascites tumor dipeptidase

Purification and properties of a mouse ascites tumor dipeptidase, a metalloenzyme.

A dipeptidase that hydrolyzes L-Ala-Gly and a wide spectrum of other L-ar-dipeptides has been purified 800-fold from the soluble fraction of Ehrlich-LettrB mouse actesis tmnor cells. The highest specific activity (micromoles of dipeptide hydrolyzed at 40” per min per mg of protein) achieved was 2,600 with Ala-Gly, the substrate with which purification was followed. With the best substrate, Ala-...

Inhibition of Ehrlich mouse ascites tumor growth by cordycepin.

Cordycepin, a nucleoside isolated from cultures of the mold Cordyceps militaris, has been demonstrated to increase the survival time of mice bearing the Ehrlich mouse ascites tumor. In all these studies the drug was administered daily for a 7-day period. AVhendrug inoculations were started the same day as tumor inoculations, inhibition of tumor growth was noted at levels of from 15 to 200 mg/kg...

The inhibition of respiration by glucose, fructose, and mannose in the Ehrlich mouse ascites tumor.

Ca2+ transport by mitochondria from L1210 mouse ascites tumor cells.

Mitochondria isolated from the ascites form of L1210 mouse leukemia cells readily accumulate Ca(2+) from the suspending medium and eject H(+) during oxidation of succinate in the presence of phosphate and Mg(2+), with normal stoichiometry between Ca(2+) uptake and electron transport. Ca(2+) loads up to 1600 ng-atoms per mg of protein are attained. As is the case in mitochondria from normal tiss...

In vitro production of mouse mammary tumor virus in a mouse mammary tumor ascites line.

An ascites line derived from a spontaneous mouse mammary carcinoma produces, on explantation and cultivation in vitro, large amounts of oncornavirus particles. The biochemical, biophysical, and electron microscopic characteristics of the virions are described. Molecular hybridization and immunological methods identify these virions as mouse mammary tumor virus.